gapdh (ABclonal Biotechnology)
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99
Structured Review
ABclonal Biotechnology
gapdh
Gapdh, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gapdh+antibody/GAPDH+Rabbit+mAb/pmc13571698-44-19-20
Average 99 stars, based on 692 article reviews
Gapdh, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gapdh+antibody/GAPDH+Rabbit+mAb/pmc13571698-44-19-20
Average 99 stars, based on 692 article reviews
gapdh - by Bioz Stars,
2026-10
99/100 stars
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Control:Article Title: Novel noscapine derivative induces AML differentiation by dual-engaging DHODH and mitochondrial lipids to trigger a metabolic–epigenetic–transcriptional cascade Article Snippet: Uridine (Sigma‒Aldrich, U3003), UDP-GlcNAc (Sigma‒Aldrich, U4375), ATRA (Sigma‒Aldrich, R2625), Vitamin D3 (MedChemExpress, HY-15398), UMP (MedChemExpress, HY-N8060), Antimycin A (Sigma‒Aldrich, A8674), Orotic acid (Sigma‒Aldrich, O8402), l -Dihydroorotic acid (Sigma‒Aldrich, D7128), Riboflavin phosphate sodium (Sigma‒Aldrich, F2253), Brequinar (MedChemExpress, HY-108325), A-485 (TargetMol, T14073 ), CTPB (Active Motif, 14065), Decylubiquinone (Cayman, 21027). .. CD11b-APC antibody (ICRF44) (Thermo Fisher Scientific Cat# 17-0118-42, RRID: AB_2016659), CD14 APC antibody (Thermo Fisher Scientific Cat# 17-0149-42, RRID: AB_10669167), CD45-APC antibody (Thermo Fisher Scientific Cat# MHCD4505, RRID: AB_10372216), DHODH antibody (Santa Cruz Biotechnology Cat# sc-166348, RRID: AB_2091729), O-GlcNAc antibody (Abcam Cat# ab2739, RRID: AB_303264), mGPDH antibody (Abcam Cat# ab188585, RRID: AB_3068580), β -Actin antibody (Cell Signaling Technology Cat# 4967, RRID: AB_330288), H3 antibody (Cell Signaling Technology Cat# 4499, RRID: AB_10544537), H3K18Ac antibody (Cell Signaling Technology Cat# 9675, RRID: AB_331550), H3K27Ac antibody (Cell Signaling Technology Cat# 8173, RRID: AB_10949503), c-Myc antibody (Cell Signaling Technology Cat# 5605, RRID: AB_1903938), Article Title: Dapagliflozin inhibits renal mesangial cell fibrosis by regulating HO-1/TGF-β1/Smad 2/3 axis. Article Snippet: Diabetic kidney disease (DKD), which is driven by multiple metabolic risk factors, can progress to renal fibrosis and ultimately end-stage renal disease, posing a serious threat to patients with diabetes.. Dapagliflozin, a competitive inhibitor of sodium-glucose cotransporter 2, has been reported to alleviate kidney damage and prevent renal failure in patients with diabetes.. However, its underlying mechanism remains incompletely understood. other:Article Title: Discovery of novel and potent harringtonine derivative P2 via systematic structure-activity Optimization: Semi-Synthesis, anti-leukemia activity, and mechanism study. Article Snippet: Natural harringtonine derivatives, isolated from cephalotaxus species, exhibit potent antiproliferative activity against hematological malignancies, particularly myeloid leukemia.. However, systematic structure-activity relationship (SAR) studies for harringtonine derivatization remain limited.. Herein, we employed nucleophilic epoxy ring-opening reactions using halogen, azido and thiophenol nucleophiles to rapidly construct a structurally diverse harringtonine derivative library. Polyacrylamide Gel Electrophoresis:Article Title: RBM15B enhancing ITGA1 mRNA stability can accelerate glioblastoma tumorigenesis via the PI3K–Akt pathway Article Snippet: RIPA Lysis Buffer (RM02998, ABclonal, China) was added to the cells for protein extraction. .. Then, after the concentrations were determined, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, blotted onto polyvinylidene fluoride membranes, blocked by 5% skim milk, and incubated with primary antibodies, including PI3K antibody (A4992, ABclonal), phospho-PI3K antibody (p-PI3K, AP0854, ABclonal), Akt antibody (A17909, ABclonal), phospho-Akt (p-Akt, AP1208, ABclonal), phospho-mTOR antibody (p-mTOR, AP1413, ABclonal), mTOR antibody (A2445, ABclonal), and Incubation:Article Title: RBM15B enhancing ITGA1 mRNA stability can accelerate glioblastoma tumorigenesis via the PI3K–Akt pathway Article Snippet: RIPA Lysis Buffer (RM02998, ABclonal, China) was added to the cells for protein extraction. .. Then, after the concentrations were determined, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, blotted onto polyvinylidene fluoride membranes, blocked by 5% skim milk, and incubated with primary antibodies, including PI3K antibody (A4992, ABclonal), phospho-PI3K antibody (p-PI3K, AP0854, ABclonal), Akt antibody (A17909, ABclonal), phospho-Akt (p-Akt, AP1208, ABclonal), phospho-mTOR antibody (p-mTOR, AP1413, ABclonal), mTOR antibody (A2445, ABclonal), and Article Title: Exosomal transfer of macrophage-derived NEAT1 enhances DNA damage response and confers cisplatin resistance in lung adenocarcinoma via the MAD1L1/p53 axis Article Snippet: .. Subsequently, primary antibodies against TSG101 antibody(Abcam, UK, ab125011); CD81 antibody (Abcam, UK, ab109201); Calnexin antibody (Abcam, UK, ab22595);p-CHK1 antibody (ab79758, Abcam, UK);p-RPA2 antibody (ab109394, Abcam, UK); Article Title: FTO-Mediated m6A Demethylation of SERPINF1 Attenuates Multiple Myeloma Progression via the Wnt/β-Catenin Pathway. Article Snippet: .. After blocking with 5% nonfat milk, membranes were incubated overnight at 4°C with the following primary antibodies: β-catenin antibody (A11512, ABclonal, China), c-Myc antibody (A1309, ABclonal), cyclin D1 antibody (A11022, ABclonal), SERPINF1 antibody (A3475, ABclonal), and Produced:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication Article Snippet: Biolabs, Massachusetts, USA; Cat#R0739L), 203 Tris-buffered saline (TBS) (Beyotime, Shanghai, China; Cat#ST663), 53 loading buffer (Abclonal, Hubei, China; Cat#RM00001), C18 StageTips (Cell Signaling Technology, Massachusetts, USA; Cat#45943), L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)–treated trypsin (Sigma-Aldrich, Missouri, USA; Cat#T1426), bovine serum albumin (BSA) (Sigma-Aldrich, Missouri, USA; Cat#B2064), TRIzol reagent (Invitrogen, California, USA; Cat#15596018), NEBNext ultra RNA library prep kit (New England Biolabs, Massachusetts, USA; Cat#E7770), nonfat dry milk (Beyotime, Shanghai, China; Cat#P0216), Tween � 20 (Sigma-Aldrich, Missouri, USA; Cat#P9416), Omni-ECL� enhanced chemiluminescence detection kit (Epizyme, Shanghai, China; Cat#SQ201L), 103 phosphate-buffered saline (PBS) (Beyotime, Shanghai, China; Cat#ST476), polybrene infection/ transfection reagent (Sigma-Aldrich, Missouri, USA; Cat#TR-1003-G), Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#L3000015), doxycycline hydrochloride (BBI Life Sciences, Shanghai, China; Cat#A600889-0025), 0.45 μm syringe filter (Thermo Fisher Scientific, Massachusetts, USA; Cat#723-2545). .. Monoclonal anti-FLAG � M2 antibody produced in mouse was used at 1:2000 dilution (Sigma-Aldrich, Missouri, USA; Cat#F1804), cross-adsorbed secondary antibody, Alexa Fluor� 488 (Thermo Fisher Scientific, Massachusetts, USA, Cat#A-11001) was used at 1:800 dilution, goat anti-mouse IgG HRP (Abmart, Shanghai, China; Cat#M21001L) was used at 1:10000 dilution, goat anti-rabbit IgG-HRP (Abmart, Shanghai, China; Cat#M21002L) was used at 1:10000 dilution, goat anti-human IgG (H + L) (Abmart, Shanghai, China; Cat#AS002) was used at 1:10000 dilution, hnRNP H1 rabbit pAb (Abclonal, Hubei, China; Cat#A5924) was used at 1:1000 dilution, hnRNP M rabbit pAb (Abclonal, Hubei, China; Cat#A6937) was used at 1:1000 dilution, hnRNP U rabbit pAb (Abclonal, Hubei, China; Cat#A3917) was used at 1:1000 dilution, hnRNP K rabbit pAb (Abclonal, Hubei, China; Cat#A1701) was used at 1:1000 dilution, Recombinant:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication Article Snippet: Biolabs, Massachusetts, USA; Cat#R0739L), 203 Tris-buffered saline (TBS) (Beyotime, Shanghai, China; Cat#ST663), 53 loading buffer (Abclonal, Hubei, China; Cat#RM00001), C18 StageTips (Cell Signaling Technology, Massachusetts, USA; Cat#45943), L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)–treated trypsin (Sigma-Aldrich, Missouri, USA; Cat#T1426), bovine serum albumin (BSA) (Sigma-Aldrich, Missouri, USA; Cat#B2064), TRIzol reagent (Invitrogen, California, USA; Cat#15596018), NEBNext ultra RNA library prep kit (New England Biolabs, Massachusetts, USA; Cat#E7770), nonfat dry milk (Beyotime, Shanghai, China; Cat#P0216), Tween � 20 (Sigma-Aldrich, Missouri, USA; Cat#P9416), Omni-ECL� enhanced chemiluminescence detection kit (Epizyme, Shanghai, China; Cat#SQ201L), 103 phosphate-buffered saline (PBS) (Beyotime, Shanghai, China; Cat#ST476), polybrene infection/ transfection reagent (Sigma-Aldrich, Missouri, USA; Cat#TR-1003-G), Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#L3000015), doxycycline hydrochloride (BBI Life Sciences, Shanghai, China; Cat#A600889-0025), 0.45 μm syringe filter (Thermo Fisher Scientific, Massachusetts, USA; Cat#723-2545). .. Monoclonal anti-FLAG � M2 antibody produced in mouse was used at 1:2000 dilution (Sigma-Aldrich, Missouri, USA; Cat#F1804), cross-adsorbed secondary antibody, Alexa Fluor� 488 (Thermo Fisher Scientific, Massachusetts, USA, Cat#A-11001) was used at 1:800 dilution, goat anti-mouse IgG HRP (Abmart, Shanghai, China; Cat#M21001L) was used at 1:10000 dilution, goat anti-rabbit IgG-HRP (Abmart, Shanghai, China; Cat#M21002L) was used at 1:10000 dilution, goat anti-human IgG (H + L) (Abmart, Shanghai, China; Cat#AS002) was used at 1:10000 dilution, hnRNP H1 rabbit pAb (Abclonal, Hubei, China; Cat#A5924) was used at 1:1000 dilution, hnRNP M rabbit pAb (Abclonal, Hubei, China; Cat#A6937) was used at 1:1000 dilution, hnRNP U rabbit pAb (Abclonal, Hubei, China; Cat#A3917) was used at 1:1000 dilution, hnRNP K rabbit pAb (Abclonal, Hubei, China; Cat#A1701) was used at 1:1000 dilution, Blocking Assay:Article Title: FTO-Mediated m6A Demethylation of SERPINF1 Attenuates Multiple Myeloma Progression via the Wnt/β-Catenin Pathway. Article Snippet: .. After blocking with 5% nonfat milk, membranes were incubated overnight at 4°C with the following primary antibodies: β-catenin antibody (A11512, ABclonal, China), c-Myc antibody (A1309, ABclonal), cyclin D1 antibody (A11022, ABclonal), SERPINF1 antibody (A3475, ABclonal), and |
